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By Springer-Verlag, Jorg Hoheisel

ISBN-10: 3540432159

ISBN-13: 9783540432159

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Singh-Gasson S, Green RD, Yue Y, Nelson C, Blattner F, Sussman MR, Cerrina F (1999) Nat Biotechnol 17:974 22. Cho RJ, Campbell MJ, Winzeler EA, Steinmetz L, Conway A, Wodicka L, Wolfsberg TG, Gabrielian AE, Landsman D, Lockhart DJ, Davis RW (1998) Mol Cell 2:65 23. Cho RJ, Huang M, Dong H, Steinmetz L, Sapinoso L, Hampton G, Elledge SJ, Davis RW, Lockhart DJ, Campbell MJ (2001) Nat Genet 27:48 24. Debouck C, Goodfellow PN (1999) Nat Genet Suppl 21:48 25. Liotta L, Petricoin E (2000) Nat Rev Genet 1:48 26.

Southern 17). Milner et al. [8] used β-globin mRNA as target and showed that duplex yield on a scanning array of anti-β-globin antisense oligonucleotides correlated with their inhibitory potential in an in vitro translation assay. We have also tested the antisense activities of over 30 oligonucleotides targeted to mRNAs of cyclins B1, B4 and B5 of Xenopus laevis in cell-free egg extracts and oocytes [17]. Scanning arrays corresponding to the first 120 nucleotides of each of the three B-type cyclins were hybridised to their corresponding transcript.

56 1 Introduction Scanning arrays are made by combinatorial in situ synthesis of a large number of oligonucleotides on a solid support in a spatially addressable fashion. They comprise sets of oligonucleotides of various lengths in which a series of oligonucleotides, complementary to the target RNA or DNA, is made by sequential coupling of nucleotides to a solid substrate (such as glass or polypropylene) that has been modified to allow oligonucleotide synthesis. The set of oligonucleotides covers every nucleotide position in the region of interest of the complement of the target.

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Chip Technology by Springer-Verlag, Jorg Hoheisel

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