By P.J. Duke, A.G. Michette
ISBN-10: 1461287774
ISBN-13: 9781461287773
ISBN-10: 1461314674
ISBN-13: 9781461314677
For numerous a long time the electron microscope has been the tool of selection for the exam of organic buildings at excessive solution. Biologists have be come conversant in the recommendations and pitfalls of pattern instruction and with the translation of the pictures got. the aim of this booklet is to introduce the biologist to a couple of new imaging suggestions which are now changing into avail in a position to complement or even expand the knowledge that may be got from the now-traditional electron microscope. a few of these innovations are nonetheless on the experimental level, whereas others, comparable to cryoelectron microscopy and confocal optical microscopy, are at complex phases of improvement and are already avail capable commercially. This publication represents a primary try and quantify the development made via carry ing jointly, in a single quantity, an account of the technical bases and the longer term potentials of some of the suggestions. even supposing the content material is essentially aimed toward biologists, microscopists in different fields must also locate the data of curiosity and use. all of the chapters are written by way of top specialists who're on the leading edge of those interesting advancements. approximately part the e-book is worried with x-ray microscopy; the editors make no apology for this considering that they're either in detail concerned with advancements linked to this box and accordingly view it, maybe with bias, as being of the maximum importance.
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Example text
Despite considerable work on the subject(82,83) the actual mechanisms giving rise to damage are not completely clear. Generally, severe radiation damage is seen after electron doses of 2000-5000 electrons per square nanometer (about an order of magnitude lower than the dose usually employed to record micrographs of biological material), which means that images recorded often have a rather poor signal-to-noise ratio because the exposure is only just above the fog level of the film. Thus, images of frozen hydrated specimens are usually not so well defined statistically (particularly at higher spatial frequencies) as material in negative stain.
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Modern Microscopies: Techniques and Applications by P.J. Duke, A.G. Michette
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