By W. J. Pledger, J. E. Estes, P. H. Howe, E. B. Leof (auth.), Jennie P. Mather (eds.)
ISBN-10: 1461593611
ISBN-13: 9781461593614
ISBN-10: 1461593638
ISBN-13: 9781461593638
The merits of acquiring a totally outlined atmosphere for the expansion of cells in vitro have been famous very early within the background of telephone tradition (Lewis and Lewis, 1911). endured curiosity within the dietary requisites of cells in vitro and in supplying an optimum setting for cells ended in the improvement of the complicated nutrient combinations on hand this present day in lots of media (Waymouth, 1972; Ham, 1965). even if, serum remained an integral part of medium for the expansion of so much telephone forms in tradition. The query of what issue (or components) in serum was once crucial for cellphone development and survival remained unanswered for numerous many years. firstly, experiments have been designed to purify the "active part" of serum for the expansion of cells in tradition. those experiments pointed out fetuin (Fisher et at., 1958) and nonsuppressible insulinlike task (Temin et at., 1972) as vital parts of serum. despite the fact that, the complexity of serum and the very low degrees of energetic elements in serum hindered development in identi fying and keeping apart serum factors.
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Additional info for Mammalian Cell Culture: The Use of Serum-Free Hormone-Supplemented Media
Example text
Once cells enter S phase they are committed to mitosis and will not arrest at any point in the cell cycle prior to cell division provided that the medium contains sufficient nutritional supplements. Thus, the biochemical events in G j that determine ability of different normal and transformed cells to regulate transit of G j phase to S phase are of particular interest in a discussion of growth regulation. , 1978). An excellent introduction to the literature on cell transformation in tissue culture is provided by Pollack (1981).
McKeehan and McKeehan (1980b) have recently suggested that serum-derived growth factors merely make nutrients and ions more available to the cell; given the appropriate medium composition, a cell would not require growth factors. Further evidence is required to generalize this concept to all cells. Transport Proteins Several proteins in blood serve as carriers for compounds essential for cell growth and survival. Hemoglobin transports oxygen, albumin binds a wide range of essential compounds such as steroid hormones or vitamins, and transferrin binds iron and facilitates its transport into cells.
In a rat hepatoma cell line, however, where insulin stimulated growth via its own high-affinity receptor, subnanomolar concentrations were active (Koontz and Iwahashi, 1981; Czech, 1982). A wide range of factors acts synergistically with insulin to stimulate DNA synthesis. , 1979), EGF (Mierzejewski and Rozengurt, 1976; Cherington 30 Paul V. , 1977; Cherington and Pardee, 1980). , 1982). , 1979). Epidermal growth factor isolated from human urine or mouse submaxillary glands stimulates DNA synthesis and growth in a variety of cell types (reviewed by Carpenter and Cohen, 1979), especially if insulin, other growth factors, or serum are present.
Mammalian Cell Culture: The Use of Serum-Free Hormone-Supplemented Media by W. J. Pledger, J. E. Estes, P. H. Howe, E. B. Leof (auth.), Jennie P. Mather (eds.)
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